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Key words: Plant source, Growth, Rainbow trout, Fry.

The Study of the Development Process of Polyspermic Eggs in the Persian Sturgeon (Acipenser persicus)

M. Karaminasab1*; M. Batebi Navai1; R.M. Nazari2

1 Department of Fisheries Sciences. University of Tehran. P.O.BOX:31585-4314, Karaj, Iran

2 Shahid Rajaee Sturgeon Hatchery Center, Sari, P.O. Box 833, Sari, Mazandaran, Iran * E-mail: M karaminasab@yahoo.com

Abstract

Sturgeons (Chondrostei, Acipenseridae) can be considered live fossils that exist in the northern hemisphere (Bemis et al., 1997). 5 species of sturgeon live in the southern part of the Caspian Sea, including; Giant Sturgeon (Huso huso), Russian sturgeon (A. gueldenstaedti), Persian sturgeon (A. persicus), Stellate (A. stellatus) and the thorn Sturgeon (A. nudiventris). These fishes are declining due to overfishing, environmental pollution and poaching (Secor et al., 2000). Since the 1990s, efforts have increased to conserve sturgeon stocks (Secor et al., 2000). Fertilization is one of the most critical stages of artificial reproduction in which an ovum is placed in contact with the activated spermatuzoids and provides fertilization arrangements and egg formation. A large number of micropyles exist in the oocyte of the sturgeon fish (Dettlaff et al., 1993). For example, the Persian sturgeon oocyte has 7/7 (Halajian et al., 1999), Russian sturgeon oocyte and stellate oocyte, respectively, 9/7 and 1/5 (Podushka, 1993), sterlyad oocyte from 5 to 13 (Dettlaff et al., 1993) and white sturgeon oocyte has 3 to 15 micropyles (Clark & Cherr, 1984). Since a large number of micropyles exist in the oocyte, the creation of polysperic eggs is quite possible. Due to the fact that Persian sturgeon is a native fish in the Caspian southern basin and most of reproduction in sturgeon farms in Iran is based on this species, the fundamental problem in breeding this species is the production of polysperic eggs. Embryology of the sturgeon fishes at the artificial propagation stage is of prime importance due to sensitive growth stages, especially in the early division of the egg cells. Accordingly, sturgeon reproduction experts must know all the different stages of embryo growth and its development from the

process of egg fertilization to the hatching stage, in order to be able to provide the necessary care during incubation. So far, the only way to identify healthy and unhealthy eggs was conducted in the second phase of mitotic division, but now with the testing and understanding of the various stages in the evolution of the polyspermic eggs, larval survival can even be calculated at the different stages of embryonic development. Simultaneously with artificial propagation of 20 pieces of breeder fish, according to common method of have been propagated artificially and after fertilization, in the second mitotic division of zygote, percentage of fertilization measured and 20 polyspermic eggs from each breeder eggs, selected and placed in separated incubators and studied in evolutionary stages as following: the third mitotic division, early gastrula (13th evolutionary stage), middle gastrula (15th evolutionary stage), small yolk plug (17th evolutionary stage), late gastrula (18th evolutionary stage), that in 26th evolutionary stage (lateral pates fuse), in 32th evolutionary stage (tip of tail of embryo touches head) and in 36th evolutionary stage (mass hatching of larvae). The results indicated that about 95% of polyspermic eggs reach to early gastrula that abnormalities have been seen ii this eggs as abnormal distribution of pigment. About 92% of these eggs reach to middle gastrula that abnormalities as un coordinated in evolution of polyspermic eggs. 84% of these eggs reach to small yolk plug. 59% of polyspermic eggs reach to late gastrula that showed the extensive mortalities. 42% of these eggs reach to26th evolutionary stage and 17% of this eggs reach to32th evolutionary stage and 7% these eggs reach to36th that alllarvae died and in comparison to monospermic larvae, have lower length and wider yolk sac and lower movement if tail.

The purpose of this survey was to investigate the development of polyspermic eggs in Persian sturgeon as a native species, and also to determine the values of the survival percentage in each of the evolution stages of polyspermic eggs, in order to enhance the reproduction of this species

Use of Artemia in larviculture of marine fish

Sairan Khani

Department of Fisheries, Faculty of Natural Resources, Urmia University, Iran Email: Sairan Khani@yahoo.com

Abstract

Live diets used in the aquaculture of fish, brine shrimp (Artemia) nauplii are the most widely used food. Over 85% of all marine animals' culture utilizes Artemia as a partial or sole diet. They have been used as a vector for the delivery of different materials, such as nutrients and probiotics. At the earliest life stages, marine fish larvae are dependent on live feed organisms, and the two most important for use in aquaculture are rotifers and brine shrimps. Artemia nauplii and rotifers are commonly used as the start feed for fish larvae worldwide. Both are non-selective, continuous filter-feeding organism, grazing on particles from the surrounding water. Brine shrimp Artemia is widely used in larviculture of a wide range of freshwater as well as marine fish either as non-hatched decapsulated cysts, freshly-hatched nauplii, or nauplii enriched with highly unsaturated fatty acids, vitamin C and E. Artemia nauplii are widely recognized as the best natural storable live feed available and are widely used in marine finfish and crustacean hatcheries around the world because of their nutritional and operational advantages. Feeding Artemia provides reasonable growth during the initial feeding stages in marine fish species in culture.

Key words: Artemia, Larviculture, Marine fish.

MHC genes application for larvae-parante assignment in captive Caspian Salmon

(Salmo trutta caspius)

Kave Khosraviani1, Mohammad Reza Kalbassi*2, Majid Sadeghizadeh3

1 Master student of Aquaculture- Tarbiar Modares University

2 Associate professor of Aquaculture- Tarbiat Modares University

3 Professor of Genetics- Tarbiat Modares University * Coresponding author:kalbassi_m@modares.ac.ir

Abstract

Reproductive success is very important in aquaculture production specielly in species that are condidate for future production. As many investigation on fishes sperm competition shows males biased competion, thus some functional genes alleles may be lossed during mixed-milt fertilization. Major Histocompatibility Compex (MHC) genes are one of the most important adaptive immune system genomes that should be conserved during artificial spawning. This issue become more critical incase of endangred species like Caspian salmon (salmo trutta caspius) that are in risk of inbreedind depression. In this study we used analysis of MHC class II beta gene (DAA locus) by single strand conformation polymorphism (SSCP) in 30 broodstocks and 50 ofsprings (F1) larves. Results indicated that some genotypes become more frequent and some of them decreased or lossed during propagation. We conclude that this genes could be good condidate for two purposes simultaneously; larvae-parante assignment and genotype assisstant selection.

Keybaords: Salmo trutta caspius, Major Histocompatibility Complex, Single Strand Conformation Polymorphism, parantage assignment

The effects of oligofructose on some growth and nutritional factors of Acipenser stellatus juvenile

Mahsa khoshbavar Rostami1, Hoseinali Khoshbavar Rostami2, Seyed Hossein Hoseinifar3

1 Department of Fisheries, Islamic Azad university of Qhaemshahr, Iran

2 Iranian fisheries research organization, inland waters research center, of Gorgan, Iran

3 Department of Fisheries, Gorgan university of agricultural sciences and natural science, Gorgan, Iran

Abstract

The aim of this study was investigation of oligofructose effects on growth and nutritional factors of Acipenser stellatus juvenile. The study included 3 treatment repeated in triplicates. Fish (29±3 g) were suppliedand stocked at density of 10 fish in tanks and fed on experimental diets for 76 days. Our results revealed significant effects on growth and nutritional and there were remarkable differences between 1 &2 % treatments and control (P < 0.05). Fish fed 1% Oligofructose had significantly lower FCR. These results showed positive effect of oligofructose on growth and nutritional factors and we suggest administration of this dose in Acipenser stellatus diet.

Keywords: oligofructose, growth factors, Acipenser stellatus

Extraction of Carotenoids from Shrimp Wastes

Amir Moghaddam Jafari

Department of Toxicology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran, Email: moghadamjafari@gmail.com

Abstract

Astaxanthin, the main carotenoid found in shrimp, its antioxidant activity was reported to be higher than that of carotene and lutein. The extraction of shrimp wastes can be used as a source of coloring and flavoring agent in marine products. The extracted carotenoids would also be a cheaper alternative than synthetic carotenoids. This study describes pigments extraction from shrimp wastes under different methods. Carotenoides were extracted and measured from shrimp waste by alkaline and enzyme. These results demonstrated that good yields can be obtained by alkaline treatment.

Key Words: Shrimp Waste, Carotenoids, Extraction Methods

Tolerance and potential adaptability of Acipenser persicus post- larvae exposed to abrupt or gradual increase of salinity

Bagher Mojazi Amiri*1, Maryam Mardaneh Khatooni1,

Seyed Hossein Hoseinifara, Jafari, V2, NoorMohammad Makhdomi3

1 Department of Fisheries, Faculty of Natural Resources, University of Tehran,31585-4314 Karaj, Iran

2 Department of Fisheries and Environmental Sciences, Faculty of, Gorgon University of Agricultural Sciences and Natural Resources, Gorgan;

3 Shahid Marjani Sturgeon Fishes Propagation and Cultivation Centre, Golestan province, Iran

* Author for correspondence: Department of Fisheries, Natural Resources Faculty, University of Tehran, Karaj, Iran. Mobile: +98 9127056413; E-mail address: bmamiri@ut.ac.ir

Abstract

One of the major aims of sturgeon culture industry is initiation of restocking programs. Determination of appropriate size, age and the location for releasing post- larvae or fingerlings is a very important task for a successful restocking program. A project including two trails was conducted to investigate the potential adaptability and tolerance ability of Persian sturgeon Acipenser persicus post- larvae (245 ± 21.53 mg) during exposure to the brackish water. The post- larvae (reared at 0.5ppt) were exposed, either abruptly or gradually to brackish water (2, 4, 6, 8, and ~12.5ppt) for five days (120 h). Daily survival rate, gill chloride cell count and variation in kidney glomeruli size were measured during exposure period in both trails. These parameters were compared at the beginning and the end of each trial. No mortality was observed during gradual adaptation to brackish water. During the gradual adaptation, chloride cell count differed significantly (P < 0.05) at 6ppt versus 12ppt. In the gradual exposure trial, glomeruli sizes displayed a reduction with salinity increasing from 8 to 12ppt.This result was observed again

between 96 and 120 h exposure to (~)12.5ppt in the abrupt exposure trial. In addition, post- larvae exposed to gradual salinity increasing had significantly higher final weight than abrupt salinity increasing. Based on our result, it seems that A. persicus post- larvae have relative salinity tolerance potential in gradual exposure.

Key words: Acipenserpersicus, salinity adaption, post- larvae, glomeruli size, Chloride cell

Review of the biogeography of Artemia Leach, 1819 (Crustacea: Anostraca) in Islamic Republic of Iran.

Amir Niknam Shiraz*1 Dr Mohammad Ali Nematollahi1 Peyman Pejman Mehr

1 Department of Fisheries, Faculty of Natural Recources, University of Tehran, Islamic Republic of Iran.

* Email: Amir Niknam@ut.ac.ir, 0919-2265344 Abstract

This review is presenting the biography of Artemia in Islamic Republic of Iran. There are many documents that show the history of studying Artemia in iran goes back to 1899 when Gunter reported Artemia in Urmia Lake.In 1976 Clark and Brown reported the Artemia Urmiana as a separate species. Iran has many natural saline lakes. One of the biggest and most important of them that unfortunately is facing the danger of drying is Urmia Lake. It is one of the largest hypersaline lakes in the world that located in Azerbaijan, northwest of Iran. Distribution of Artemia in Iran in addition to Urmia Lake have reported in lakes of Qom, Jazmourian, Parishan, Maharloo and Bakhtegan and Gavkhooni lagoon and this distribution includes different parts of north, centre and south of the country but most of these lakes are recently dried or are drying.Our focus in this review is in Artemia urmiana and its natural habitat Urmia lake.

Key Words: Artemia, Urmia Lake, Biography, Islamic Republic of Iran

Application Of Freshwater Rotifer To Sand Goby (Oxyeleotris Marmoratus) Fries

Tran Suong Ngoc, Vu Ngoc Ut and Nguyen Van Hoa Abstract

Studies aim to determine a possibility to apply freshwater rotifer (Brachionus angularis) in nursery of sand goby (Oxyeleotris marmoratus)from newly hatched larvae up to 10-day-old. The first experiment to detect the optimal rotifer density for feeding to larvae of marble goby through four different rates (treatment)of 5, 8, 11 ind./mL while control was fed with mixture of yolk and soya meal. The second experiment to find out the proper density of Chlorella applied into nursing system of sand goby under four appropriate algal density: 0; 0.5; 1 and 1.5 cell/mL. Results indicated that fish larval fed with 5 ind./mL was not significantly different to the control, however when leveled up rotifer into 11 ind./mL could improve fish larval survival on day 10 from 19,9±1,4% to 35,3+5,7%. More interesting as their survival continued to increase up to 43,6+2,8% when the system was added Chlorella at 1,5x106 cell/mL and to maintain rotifer at the density of 11 ind./mL.

Key words: fresh water rotifer (Brachionus angularis), Chlorella, sand goby (Oxyeleotris marmoratus)

Effects of partial replacement of fishmeal by processed

soybean meal (HP 300) ™ on some hematological indices of juvenile Siberian sturgeon (Acipenser baerii)

Mehrnaz Safarzadehnia1*, Mehrdad Nasri Tajan2, Rezvanollah Kazemi3, Reza Taati4

1 Department of Fisheries, Science and Research Branch, Islamic Azad University, Guilan, Iran.

2 Department of Fisheries, Bandar Anzali Branch, Islamic Azad University, Bandar Anzali, Iran.

3 International Sturgeon Research Institute. Rasht, Iran.

4 Department of Fisheries, Talesh Branch, Islamic Azad University, Talesh, Iran.

Abstract

An 8-week feeding experiment was conducted to determine the potential use of processed soybean meal (HP 300) ™ as a partial replacement of fishmeal in the diet of juvenile Siberian sturgeon. A total of 72 juveniles of A.baerii weighing 198.25 + 17.93 g were randomly distributed into 12 fiberglass tanks (500 L) and kept at a density of 6 fish per tank. Diets were formulated to include 0, 10, 20 and 30%) (Diets: D0, D10, D20 and D30, respectively) of fishmeal protein being substituted by processed soybean meal. At the end of the feeding trial, blood sampling was conducted. Levene's test was used to determine the homogeneity of variance. The means of all parameters were subjected to one-way ANOVA and comparisons among treatment means were made by Tukey's test using SPSS. According to findings of this study, there were no significant differences in red blood cell (RBC), white blood cell (WBC), hematocrit (PCV), MCV, MCH and MCHC in fish fed diets with different processed soybean meal replacement levels but weight gain and haemoglobin (Hb) showed significant differences among the treatments. The highest weight gain and haemoglobin values were recorded in D30

and D0, respectively. WBC, RBC and MCV were higher in D10, D20

and D30 respectively in comparison with the other treatments.

Based on the obtained results, it can be declared that the different levels of processed soybean meal cannot improve the hematological indices of Siberian sturgeon but it can be economically suitable for rearing because of the enhancement of growth and its inexpensive price.

Keywords: Siberian sturgeon (Acipenser baerii), Processed soybean meal (HP 300) ™ and Hematological indices.

The correlation between levels of dietary nucleotides and Fatty Acid Profile of rainbow trout (Oncorhynchus mykiss) fingerling

Naeemeh Salami Khorshidi1, Saeed Keyvanshokooh2, Amir Parviz Salati3, Mohammad Zakeri4, Nematollah Mahmoudi5

1 M.sc in Aquaculture, Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Email: n.salimi88@yahoo.com

2 Assistant Professor of Fisheries Department, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Email: keyvan56@yahoo.com

3 Assistant Professor of Fisheries Department, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Email: salatia@gmail.com

4 Assistant Professor of Fisheries Department, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Email: mhdzakeri@yahoo.com

5 Ph.D. Student of Fisheries Department, Faculty of Marine Sciences, Tarbiat Modares University, Mazandaran, Noor, Email: mahmoudi.nemat@gmail.com

Abstract

Nucleotides are the building blocks of DNA and RNA. And are crucial to the storage, transfer and expression of genetic information. Although the roles of nucleotides administered exogenously have been debated for many years, their potential application in aquaculture has received heightened attention in recent years. Acording to observation made in recent years, Dietary nucleotide increase in absorption digestive system and influence on lipid and protein metabolism. The present study was conducted to investigate the effects of dietary nucleotides (NT) on proximate analysis of rainbow trout with average weight 11.35+0.32 during 8 weeks. This experimental was carried out in 700 L circular tanks with 40 fish per tank. NT was added to the diet at a rate of 0.0, 0.05, 0.1, 0.15 and 0.2 percent. Fish were fed 5 time daily at a rate of 3-5% body

weight per day. The results showed that, EPA and Linolenic acid levels increased in fish fed on 0.2% NT supplemented diet compared to the control group. No significant differences (p> 0.05) were observed in polyunsaturated fatty acids (PUFA), n-3, n-6, n3/n6 and saturated fatty acids (SFA) content among the test groups. Correlation analysis showed a significant association between EPA and NT are (05/0> P) and correlation 0/ 64 was. The results suggest that dietary nucleotides exerted positive effects on fatty acid composition in rainbow trout.

Key words: Nucleotide, correlation, fatty acid composition, rainbow trout, Oncorhynchus mykiss

The correlation between levels of dietary nucleotides and amino acid profile of rainbow trout

(Oncorhynchus mykiss) muscle

Naeemeh Salami Khorshidi1, Saeed Keyvanshokooh2, Amir Parviz Salati3, Mohammad Zakeri4, Nematollah Mahmoudi5

1 M.sc in Aquaculture, Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Email: n. salimi8 8 @ yahoo.com

2 Assistant Professor of Fisheries Department, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Email: keyvan56@yahoo.com

3 Assistant Professor of Fisheries Department, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Email: salatia@gmail.com

4 Assistant Professor of Fisheries Department, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Email: mhdzakeri@yahoo.com

4 Ph.D. Student of Fisheries Department, Faculty of Marine Sciences, Tarbiat Modares University, Mazandaran, Noor, Email: mahmoudi.nemat@gmail.com

Abstract

Trout are an efficient fish with respect to converting diet ingredients not consumed by humans into human food. Trout yield more than 50% of edible product after processing, and this product is high in essential fatty acids and protein and low in saturated fats relative to animal protein. Over the past two decades trout diets have changed in several ways. Acording to observation made in recent years, Dietary nucleotide increase in absorption digestive system and influence on lipid and protein metabolism. The present study was conducted to investigate the effects of dietary nucleotides (NT) on muscle amino acid profile of rainbow trout with average weight 11.35+0.32 during 8 weeks. This experimental was carried out in 700 L circular tanks with 40 fish per tank. NT was added to the diet at a rate of 0.0, 0.05, 0.1, 0.15 and 0.2 percent. Fish were fed 5

time daily at a rate of 3-5% body weight per day. The results showed that, dietary supplementation of NT has significant effect on His, Arg, Thr, Val, Ilu, Leu, Phe, Lys, Glu, Ser, Gly. Tyr+Phe, TEAA and TNEAA. In fish fed 0.2% NT, total essential amino acid (EAA) content was significantly higher among the experimental groups. Correlation analysis showed a significant association between His, Arg, Thr, Ser, Tyr+Phe and NT are (05/0> P) and correlation 0/64 was. The results suggest that dietary nucleotides exerted positive effects on amino acid composition in rainbow trout.

Key words: Nucleotide, correlation, amino acid composition, rainbow trout, Oncorhynchus mykiss

The correlation between levels of dietary nucleotides and biochemical composition of rainbow trout

(Oncorhynchus mykiss)

Naeemeh Salami Khorshidi1, Saeed Keyvanshokooh2, Amir Parviz Salati3, Mohammad Zakeri4, Nematollah Mahmoudi5

1 M.sc in Aquaculture, Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Email: n. salimi8 8 @ yahoo.com

2 Assistant Professor of Fisheries Department, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Email: keyvan56@yahoo.com

3 Assistant Professor of Fisheries Department, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Email: salatia@gmail.com

4Assistant Professor of Fisheries Department, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Email: mhdzakeri@yahoo.com

5 Ph.D. Student of Fisheries Department, Faculty of Marine Sciences, Tarbiat Modares University, Mazandaran, Noor, Email: mahmoudi.nemat@gmail.com

Abstract

Nucleotides are low molecular weight intracellular compounds which play key roles in diverse physiological and biochemical functions including encoding genetic information and mediating energy metabolism. Acording to observation made in recent years, Dietary nucleotide increase in absorption digestive system and influence on lipid and protein metabolism. The present study was conducted to investigate the effects of dietary nucleotides (NT) on Body composition of rainbow trout with average weight 11.35+0.32 during 8 weeks. This experimental was carried out in 700 L circular tanks with 40 fish per tank. NT was added to the diet at a rate of 0.0, 0.05, 0.1, 0.15 and 0.2 percent. Fish were fed 5 time daily at a rate of 3-5% body weight per day. At the end of feeding trial, three representative fish from each tank were obtained randomly, then fish were homogenized and subjected to proximate

analyses to determine whole-body compotion including moisture, protein, lipid and ash. The results showed that, the fish fed 0.2% NT, higher whole body protein, and lower lipid and ash levels compared to the control group. Correlation analysis showed a significant association between dietary protein and NT are (05/0> P) and correlation 0/ 79 was. Administration of dietary NT at the level of 0.2% seems to positively influence on proximate composition of fingerling rainbow trout.

Key words: Nucleotide, correlation, proximate analysis, Rain bow trout,

Oncorhynchus mykiss

Enrichment mechanisms of Artemiafranciscanawith probiotic bacteria -for use in marine fish larviculture

Saeed Ziaei-Nejad1, Gholamraza Rafiee2, Alireza Mirvaghefi2, Hamid Farahmand2

1Department of Fisheries, Natural Resources Faculty, BehbahanKhatamalanbia

University of Technology, Behbahan, Iran. 2 Department of Fisheries, Natural Resources Faculty, University of Tehran, Karaj, Iran.

Abstract

This study examined the ability of two enrichment mechanisms, bioencapsulation or/and attachment to the body surface, of Artemianauplii (Artemiafranciscana) with two isolated probiotics. The probiotics tested were Bacillus subtilis BP6 and Lactobacillus plantarium LP3 delivered at two concentrations (103 and 106 cells/ml) and two growth phases (start of exponential and stationary phases). Results show that both bacteria were able to bioencapsulate into and attached to Artemia successfully. This study showed that in general, the attachment of probiotic to the Artemia increased, while bioencapsulated probiotic to Artemia decreased over the time in all treatments. A maximum attachment of 2.6+0.2 x 103 CFU/nauplii for isolate B.subtilis BP6 and 1.7+0.1 x 103 CFU/nauplii for isolate L.plantarum LP3 occurred when nauplii were exposed to the probiotic bacteria at 106 cells/ml in the stationary phase. In comparison, maximum bioencapsulation occurred with 4.3+0.2 x 103 CFU/nauplii for isolate B.subtilis BP6 and 2.4+0.1 x 103 CFU/nauplii for isolate L.plantarum LP3 when nauplii were exposed to the probiotic bacteria at 106 cells/ml in both the exponential and stationary phases.

Keywords: Probiotic; Artemiafranciscana; enrichment; bioencapsulation; Bacillus subtilis; Lactobacillus plantarium

A survey of blood parameters in ship

(Acipenser nudiventris) broodstocks under rearing

condition

Zohrabi, M.1; Nasri Tajan, M.*2; Kazemi, R.3; Bagherzadeh Lakani, F4, Pourdehghani. M3.

1 Department of Fisheries, Islamic Azad University, Science&Research Branch, Guilan, Iran.

2 Department of Fisheries, Islamic Azad University, Anzali Branch, Anzali, Iran.

3 International Sturgeon Research Institute, Rasht, Iran.

4 Department of Fisheries, Faculty of Natural Resources, Urmia University, Iran * Corresponding author's E-mail: Nasri_mehrdad@yahoo.com

Abstract

Sturgeons declining populations in their native habitats, mainly the Caspian Sea and most species of them are currently considered to be at risk of extinction, making them more critically endangered. Sturgeons mature very late in life and so the broodstocks have high value in aquaculture of these species. Five female with mean initial body weight and total length of 11810+1479.4g and 126.1+3.2 cm and five male with mean initial body weight and total length of 7120+ 800.9 g and 110.3+4 cm were provided form International Sturgeon Research Institute (Rasht, Guilan province, Iran). Approximately, 2 ml of blood were taken from the caudal vein or artery of each fish using a 5 ml heparinized syringe. Test tubes containing blood were centrifuged for 5 min at 7000 rpm. Haematocrite (Hct), hemoglobin (Hb), red blood cell counts (RBC), mean corpuscular volume (MCV), mean cellular haemoglobin (MCH) or mean cell haemoglobin concentration (MCHC) was measured. data collected from the experiments were subjected to student's t-test to determine differences using SPSS 16. Kolmogorov - Smirnov Z test was applied to check the data normality. Results showed no significant differences in blood parameters between male and female broodstocks of Ship (Acipenser nudiventris) (P>0.05).

Keywords: Acipenser nudiventris, Ship, Female, Male, Blood parameters.

A survey of blood parameters in Acipenser stellatus broodstocks under rearing condition

Zohrabi, M. 1; Nasri Tajan, M.* 2; Kazemi, R. 3; Bagherzadeh Lakani, F 4 and Pourdehghani.M3.

1 Department of Fisheries, Islamic Azad University, Science&Research Branch, Guilan, Iran.

2 Department of Fisheries, Islamic Azad University, Anzali Branch, Anzali, Iran.

3 International Sturgeon Research Institute, Rasht, Iran.

4 Department of Fisheries, Faculty of Natural Resources, Urmia University, Ira * Corresponding author's E-mail: Nasri_mehrdad@yahoo.com

Abstract

Sturgeons are slow-growing and mature very late in life. In Caspian Sea most sturgeon species will be extinct in the near future in result of some threats including pollution and habitat fragmentation, over-fishing for meat and caviar production, destruction of their spawning grounds and Water pollution. Five female with mean initial body weight and total length of 5075 + 370.5 g and 123.6 + 3.9 cm and five male with mean initial body weight and total length of 5380 + 623.2g and 116 + 4.5 cm were provided form International Sturgeon Research Institute (Rasht, Guilan province, Iran). Approximately, 2 ml of blood were taken from the caudal vein of each fish using a 5 ml heparinized syringe. Test tubes containing blood were centrifuged for 5 min at 7000 rpm. Haematocrite (Hct), hemoglobin (Hb), red blood cell counts (RBC), mean corpuscular volume (MCV), mean cellular haemoglobin (MCH) or mean cell haemoglobin concentration (MCHC) was measured. Data collected from the experiments were subjected to student's t-test to determine differences using SPSS 16. At first, Kolmogorov - Smirnov Z test was applied to check the data normality. When a significant main difference was found, the differences between treatment means were determined by Tukey's tests as a post-hoc test. Significant differences were found in Hct and RBC (P<0.05) but in other parameters no significant difference were observed (P>0.05).

Key words: Acipenser Stellatus, Female, Male, Blood parameters.

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